Phase Contrast Microscopy Images
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The phase contrast microscopy images below display a technique using special phase contrast objectives and phase contrast condenser achieving an improved contrast using light microscopy when viewing transparent/colorless species or specimens.  Cells and their components are hard to visualize using a light microscope but phase contrast allows for cells to be studied "live" without needing to be fixed or stained.




Anthrax Bacillus anthracis, Phase Contrast Microscopy Usage:public domain (CC0), unaltered Author:Larry Stauffer, Oregon State Public Health Laboratory, USCDCPAnthrax Bacillus anthracis, Phase Contrast Microscopy Usage:public domain (CC0), unaltered Author:Larry Stauffer, Oregon State Public Health Laboratory, USCDCP

Various phosphatidylcholine liposomes in suspension. Method of phase contrast microscopy (1000-fold magnification) by ArkhipovSergey / CC BY-SA (https://creativecommons.org/licenses/by-sa/4.0)Various phosphatidylcholine liposomes in suspension. Method of phase contrast microscopy (1000-fold magnification) by ArkhipovSergey / CC BY-SA (https://creativecommons.org/licenses/by-sa/4.0)

Pleurotus ostreatus 400x spores - Phase contrast microscopy. https://mushroomobserver.org/129477by Alan Rockefeller / CC BY-SA (https://creativecommons.org/licenses/by-sa/3.0)Pleurotus ostreatus 400x spores - Phase contrast microscopy. https://mushroomobserver.org/129477by Alan Rockefeller / CC BY-SA (https://creativecommons.org/licenses/by-sa/3.0)

Micrograph of Whatman lens tissue paper. Phase contrast illumination. 10x magnification, 1.559 μm/px by Zephyris / CC BY-SA (https://creativecommons.org/licenses/by-sa/3.0)Micrograph of Whatman lens tissue paper. Phase contrast illumination. 10x magnification, 1.559 μm/px by Zephyris / CC BY-SA (https://creativecommons.org/licenses/by-sa/3.0)

Phase Contrast Microscopy of Dunaliella - Green chlorophyte microbial alga that abounds in Lake Mono, California by djpmapleferryman on Flickr.com, image unaltered. CCO.Phase Contrast Microscopy of Dunaliella - Green chlorophyte microbial alga that abounds in Lake Mono, California by djpmapleferryman on Flickr.com, image unaltered. CCO.

Light microscopy, variable phase contrast.Colonial peritrich ciliate Ophrydium by Лаборатория "Микрокосмос" - Own work, CC BY-SA 4.0, https://commons.wikimedia.org/w/index.php?curid=64716942Light microscopy, variable phase contrast.Colonial peritrich ciliate Ophrydium by Лаборатория "Микрокосмос" - Own work, CC BY-SA 4.0, https://commons.wikimedia.org/w/index.php?curid=64716942


Also see: Electron Microscopy images, Microscopy Imaging Techniques

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